Chitin-glucan improves important pathophysiological features of irritable bowel syndrome

Figure 5 Modulation of analgesic-related receptors in HT-29 cells incubated with chitin-glucan at different concentrations, with or without lipopolysaccharide stimulation. A: Modulation of analgesic-related receptors in non-stimulated HT-29 cells incubated for 3 h in medium alone (medium), chitin-glucan at 500 microgram per milliliter [chitin glucan (CG) 500, grey], or chitin-glucan at 1000 microgram per milliliter (CG 1000, dark grey); B: Modulation of analgesic-related receptors in HT-29 cells stimulated during 24 h with lipopolysaccharide (LPS) and incubated 3 additional hours in medium alone (LPS), chitin-glucan at 500 microgram per milliliter (LPS + CG: 500, white), or chitin-glucan at 1000 microgram per milliliter (LPS + CG: 1000, black). ^aP < 0.05, ^bP < 0.01. MOR: Mu-opioid receptor; CB2: Cannabinoid receptor; CG: Chitin glucan; LPS: Lipopolysaccharide.