OSW-1 triggers necroptosis in colorectal cancer cells through the RIPK1/RIPK3/MLKL signaling pathway facilitated by the RIPK1-p62/SQSTM1 complex

Figure 7 The inhibition of colorectal cancer cell proliferation is associated with necroptosis induced by OSW-1 in vivo. A: Treatment schedule for mice intravenously injected with 5 × 10⁶ HT29 cells; B: Images of the harvested subcutaneous tumors. Tumor growth in the OSW-1 group was markedly suppressed compared to that in the control group; C: The tumor volume (mm³) and weight in the high-dose OSW-1 group were lower than those in the low-dose group; D: Immunohistochemistry staining of p-RIPK1 and p-MLKL in xenograft nude mice (magnification, 200 ×); E: In xenograft nude mouse models, the expression levels of RIPK1, RIPK3 and MLKL in the high-dose group were increased in comparison to the low-dose group. ^aP < 0.05 and ^bP < 0.01 vs Control, ^cP < 0.05 and ^dP < 0.01 vs Low dose group.