OSW-1 triggers necroptosis in colorectal cancer cells through the RIPK1/RIPK3/MLKL signaling pathway facilitated by the RIPK1-p62/SQSTM1 complex

Figure 6 p62/SQSTM1 regulated OSW-1-induced necroptosis in colorectal cancer cells. A: The mRNA expression levels of p62/SQSTM1 and RIPK1 in HT29 and HCT116 cells were assessed after exposure to OSW-1 and p62/SQSTM1 siRNA; B: The protein expression levels of necroptosis-related proteins in HT29 and HCT116 cells were evaluated after treatment with OSW-1, with or without the addition of p62/SQSTM1 siRNA; C: A Hoechst 33342/propidium iodide dual staining assay was used to assess necroptosis in HT29 and HCT116 cells following exposure to OSW-1, with or without the addition of p62/SQSTM1 siRNA. ^bP < 0.01 vs Control, ^cP < 0.05 and ^dP < 0.01 vs OSW-1, ^fP < 0.01 vs sip62. Each data point represents the mean ± SE. PI: Propidium iodide.