OSW-1 triggers necroptosis in colorectal cancer cells through the RIPK1/RIPK3/MLKL signaling pathway facilitated by the RIPK1-p62/SQSTM1 complex

Figure 4 OSW-1 induced necroptosis in colorectal cancer cells through the RIPK1/RIPK3/MLKL pathway. A: Cell Counting Kit-8 assay was used to assess the viability of HT29 and HCT116 cells treated with OSW-1 and different concentrations of necrostatin-1 (Nec-1) for 24 h; B: The expression levels of necroptosis-related proteins in HT29 and HCT116 cells after exposure to OSW-1 for 24 h with the addition of Nec-1; C: Assessment of mitochondrial function via JC-1 staining of HT29 and HCT116 cells after exposure to OSW-1 for 24 h with the addition of Nec-1; D: A Hoechst 33342/PI dual staining assay was used to examine cell necroptosis after 24 h of treatment with OSW-1 and Nec-1. Scale bar = 50 μm; E: Lactate dehydrogenase release was used to assess the cell death rate of HT29 and HCT116 cells exposed to OSW-1 and Nec-1 for 24 h. ^bP < 0.01 vs Control, ^cP < 0.05 and ^dP < 0.01 vs OSW-1. Each data point represents the mean ± SE. PI: Propidium iodide; Nec-1: necrostatin-1.