OSW-1 triggers necroptosis in colorectal cancer cells through the RIPK1/RIPK3/MLKL signaling pathway facilitated by the RIPK1-p62/SQSTM1 complex

Figure 3 OSW-1 triggers necroptosis in colorectal cancer cell culture. A: Necrotic morphological changes were observed in HT29 and HCT116 cells treated with OSW-1 using optical microscopy (400 × magnification). Necrotic cells are indicated by red arrows; B: Typical morphological changes associated with necroptosis were identified in HT29 and HCT116 cells treated with OSW-1 through TEM (3000 × magnification); C: The expression levels of proteins associated with necroptosis in HT29 and HCT116 cells following 24 h of exposure to OSW-1; D: A Hoechst 33342/propidium iodide dual staining assay was used to assess the rate of necroptosis in HT29 and HCT116 cells following treatment with OSW-1. Scale bar = 50 μm. ^aP < 0.05 and ^bP < 0.01 vs Control. Each data point represents the mean ± SE. PI: Propidium iodide.