OSW-1 triggers necroptosis in colorectal cancer cells through the RIPK1/RIPK3/MLKL signaling pathway facilitated by the RIPK1-p62/SQSTM1 complex

Figure 1 OSW-1 suppressed HT29 and HCT116 cell survival. A: Chemical structure of OSW-1; B: Cell counting kit-8 assay was used to access the viability of HT29 and HCT116 cells treated with different concentrations of OSW-1 for 24 h; C: Lactate dehydrogenase release assays were used to assess the cell death rate of HT29 and HCT116 cells treated with different concentrations of OSW-1 for 24 h; D: A colony formation assay was used to evaluate the clonogenic survival of HT29 and HCT116 cells following treatment with OSW-1; E: Flow cytometry with Annexin V-FITC/propidium iodide double staining was used to assess the percentage of apoptotic and necrotic HT29 and HCT116 cells after OSW-1 treatment; F: JC-1 staining was used to assess mitochondrial function in HT29 and HCT116 cells after OSW-1 treatment. ^bP < 0.01 vs Control, ^cP < 0.05 vs OSW-1 (1 μM). Each data point represents the mean ± SE. LDH: Lactate dehydrogenase; PI: Propidium iodide.