Comparative transcriptomic analysis reveals the molecular changes of acute pancreatitis in experimental models

Figure 2 RNA-seq identified the molecular changes during the development of acute pancreatitis. Comparative analysis of the caerulein (CAE) group and CAE + lipopolysaccharide (LPS) group with the control (Con) group. A: Volcano plot of significant differentially expressed (SDE) genes (P value < 0.05 and |log2FC| > 1), with red indicating significant upregulation and green indicating significant downregulation; B: Venn diagram, where blue represents CAE group vs Con group, and red represents CAE + LPS group vs Con group, with the gray area representing commonly differentially expressed genes in both groups compared to the Con group; C: Heatmap displaying the expression of SDE genes in each pancreatic sample, with red indicating upregulated genes and blue indicating downregulated genes; D: Gene Ontology (GO) functional annotation of the top 30 significantly different genes, with the GO classification chart showing the distribution of entries related to biological processes, cellular components, and molecular functions; E: Enrichment analysis results of the top 20 significantly different Kyoto Encyclopedia of Genes and Genomes pathways; F: Gene Set Enrichment Analysis for the NOD-like receptor, TLR, NF-κB, and P53 signaling pathways, as well as apoptosis. CAE: Caerulein; CON: Control; LPS: Lipopolysaccharide.