ALKBH5 suppresses autophagic flux via N6-methyladenosine demethylation of ZKSCAN3 mRNA in acute pancreatitis

Figure 5 ALKBH5 promoted the expression of ZKSCAN3 and inhibited autophagic flux. A: After interfering with ALKBH5 expression, the expression level of ZKSCAN3 mRNA was detected by qPCR; B: The expression levels of ZKSCAN3 protein and autophagy related proteins were detected by western blot; C: Autophagic microstructure was observed by transmission electron microscopy; D: LC3 was detected by immunofluorescence (magnification × 800); E: LAMP-2 was detected by immunofluorescence (magnification × 800). ^aP < 0.05 vs negative cerulein group. NC: Negative control.