ALKBH5 suppresses autophagic flux via N6-methyladenosine demethylation of ZKSCAN3 mRNA in acute pancreatitis

Figure 4 The expression and function of ALKBH5 in acute pancreatitis. A: qPCR was used to detect ALKBH5 mRNA expression in acute pancreatitis (AP) group and control group; B: The expression level of the ALKBH5 protein in AP group and control group was detected by western blot; C: qPCR was used to detect ALKBH5 mRNA expression in the MPC-83 cell line treated with different siRNAs; D: western blot was used to detect ALKBH5 protein expression in MPC-83 cell line treated with three different siRNA; E: After interfering with the expression of ALKBH5, the expression level of inflammatory factors in the mouse AP cell line was detected by ELISA. ^aP < 0.05 vs saline group; ^bP < 0.05 vs negative control group; ^cP < 0.05 vs cerulein group. NC: Negative control; TNF: Tumor necrosis factor; IL: Interleukin.