Alkaline sphingomyelinase deficiency impairs intestinal mucosal barrier integrity and reduces antioxidant capacity in dextran sulfate sodium-induced colitis

doi:10.3748/wjg.v30.i10.1405

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Mar 14, 2024 (publication date) through Jul 21, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 14, 2024; 30(10): 1405-1419
Published online Mar 14, 2024. doi: 10.3748/wjg.v30.i10.1405

Table 2 Quantitative real-time reverse transcription-polymerase chain reaction primers for target genes

Gene	Gene ID	Primer
ZO-1 F	21872	AGCTGCCTCGAACCTCTACTCTAC
ZO-1 R	21872	GCCTGGTGGTGGAACTTGCTC
Occludin F	18260	TGCTTCATCGCTTCCTTAGTAA
Occludin R	18260	GGGTTCACTCCCATTATGTACA
Claudin2 F	12738	GGGCAATCGTACCAACTA
Claudin2 R	12738	CAGTCAGGCTGTATGAGTTG
Claudin3 F	12739	GGCGGCTCTGCTCACCTTA
Claudin3 R	12739	CGTACAACCCAGCTCCCATC
Claudin5 F	12741	TGGTGCTGTGTCTGGTAGGATGG
Claudin5 R	12741	GTCACGATGTTGTGGTCCAGGAAG
Claudin8 F	54420	TGTCTGCCTTCATCGAAAGTAA
Claudin8 R	54420	GGCATGCCTCATACAATTCATC
MUC2 F	17831	TGCTGACGAGTGGTTGGTGAATG
MUC2 R	17831	TGATGAGGTGGCAGACAGGAGAC
Nrf2 F	18024	CGAGATATACGCAGGAGAGGTAAGA
Nrf2 R	18024	GCTCGACAATGTTCTCCAGCTT
HO-1 F	15368	ACCGCCTTCCTGCTCAACATTG
HO-1 R	15368	CTCTGACGAAGTGACGCCATCTG
GSH-Px F	14775	AGGGCTGTGCTGATTGAGAATGTG
GSH-Px R	14775	CTCCTGATGTCCGAACTGGTTGC
GCLc F	14629	ATGTGGACACCCGATGCAGTATT
GCLc R	14629	TGTCTTGCTTGTAGTCAGGATGGTTT
SOD2 F	20656	TCCCAGACCTGCCTTACGACTATG
SOD2 R	20656	CTCCTCGGTGGCGTTGAGATTG
GAPDH F	14433	GGTTGTCTCCTGCGACTTCA
GAPDH R	14433	TGGTCCAGGGTTTCTTACTCC

F: Forward; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; GCLc: Glutamate-cysteine ligase catalytic subunit; GSH-Px: Glutathione peroxidase; HO-1: Heme oxygenase-1; MUC2: Mucin 2; Nrf2: Nuclear factor erythroid 2-related factor 2; R: Reverse; SOD: Superoxide; ZO-1: Zonula occludens-1.

Citation: Tian Y, Li X, Wang X, Pei ST, Pan HX, Cheng YQ, Li YC, Cao WT, Petersen JDD, Zhang P. Alkaline sphingomyelinase deficiency impairs intestinal mucosal barrier integrity and reduces antioxidant capacity in dextran sulfate sodium-induced colitis. World J Gastroenterol 2024; 30(10): 1405-1419
URL: https://www.wjgnet.com/1007-9327/full/v30/i10/1405.htm
DOI: https://dx.doi.org/10.3748/wjg.v30.i10.1405