Crohn’s disease as the intestinal manifestation of pan-lymphatic dysfunction: An exploratory proposal based on basic and clinical data

Figure 4 Zebrafish as a good candidate model for the study of Crohn’s disease. A: Schematic diagram showing the morphology of zebrafish embryo at 5 dpf. The solid yellow line indicates the intestine of the embryo; B: Morphology of zebrafish embryo visualized by hematoxylin and eosin staining of embryonic sections at 5 dpf. Sections were cut along the sagittal plane. The dotted line indicates the whole intestine; C: Fluorescence signals of ET33J1: enhanced green fluorescent protein zebrafish reporter (top) and dichloro-dihydro-fluorescein diacetate treatment (bottom) showing the morphology of the whole intestines at 5 dpf (top) and 7 dpf (bottom). Scale bar: 200 µm. dpf: Days post fertilization.