Mechanistic research: Selenium regulates virulence factors, reducing adhesion ability and inflammatory damage of Helicobacter pylori

doi:10.3748/wjg.v30.i1.91

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Jan 7, 2024 (publication date) through Nov 27, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Gastroenterol. Jan 7, 2024; 30(1): 91-107
Published online Jan 7, 2024. doi: 10.3748/wjg.v30.i1.91

Table 1 Primer list

Name	Sequence	Company performing synthesis
CagA	F: ACCCCTAGTCGGTAATG	Shanghai Invitrogen Biotech Co., Ltd.
CagA	R: GCTTTAGCTTCTGATACTGC	Shanghai Invitrogen Biotech Co., Ltd.
VacAs1a	F: GTCAGCATCACACCGCAAC	Shanghai Generay Biotech Co., Ltd.
VacAs1a	R: CTGCTTGAATGCGCCAAAC	Shanghai Generay Biotech Co., Ltd.
16sRNA	F: CTGGAGAGACTAAGCCCTCC	Shanghai Invitrogen Biotech Co., Ltd.
16sRNA	R: AGGATCAAGGTTTAAGGATT	Shanghai Invitrogen Biotech Co., Ltd.
IL-6	F: GCAGAAAAAGGCAAAGAATC	Wuhan Genecreate Biotech Co., Ltd.
IL-6	R: CTACATTTGCCGAAGAGC	Wuhan Genecreate Biotech Co., Ltd.
IL-8	F: CACCGGAAGGAACCATCTCA	Wuhan Genecreate Biotech Co., Ltd.
IL-8	R: TGGCAAAACTGCACCTTCACA	Wuhan Genecreate Biotech Co., Ltd.
TNF-α	F: TCTTCTCGAACCCCGAGTGA	Wuhan Genecreate Biotech Co., Ltd.
TNF-α	R: CCTCTGATGGCACCACCAG	Wuhan Genecreate Biotech Co., Ltd.
GAPDH	F: GGACCTGACCTGCCGTCTAG	Wuhan Genecreate Biotech Co., Ltd.
GAPDH	R: GTAGCCCAGGATGCCCTTGA	Wuhan Genecreate Biotech Co., Ltd.
Cag1	F: GCTATGGGGATTGTTGGGATAA	Shanghai Sangon Biotech Co., Ltd.
Cag1	R: GCTTCAGTTGGTTCGTTGGTAA	Shanghai Sangon Biotech Co., Ltd.
Cag3	F: GACACCTTGAATGTGAATGACAAA	Shanghai Sangon Biotech Co., Ltd.
Cag3	R: GTTGTAATACCCATTGACTTGCTCTAA	Shanghai Sangon Biotech Co., Ltd.
CagT	F: TCTAAAAAGATTACGCTCATAGGCG	Shanghai Sangon Biotech Co., Ltd.
CagT	R: CTTTGGCTTGCATGTTCAAGTTGCC	Shanghai Sangon Biotech Co., Ltd.
CagE	F: GCGATTGTTATTGTGCTTGTAG	Shanghai Sangon Biotech Co., Ltd.
CagE	R: GAAGTGGTTAAAAAATCAATGCCCC	Shanghai Sangon Biotech Co., Ltd.
CagM	F: ACAAATACAAAAAAGAAAAAGAGGC	Shanghai Sangon Biotech Co., Ltd.
CagM	R: ATTTTTCAACAAGTTAGAAAAAGCC	Shanghai Sangon Biotech Co., Ltd.

16sRNA: 16S ribosomal RNA; CagA: Cytotoxin-associated protein A; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; IL-6: Interleukin 6; TNF-a: Tumor necrosis factor-alpha; VacAs1a: Vacuolating cytotoxin gene As1a.

Citation: Qin C, Huang GR, Guan AX, Zhou WT, Chen H, Luo PP, Luo XK, Huang YQ, Huang ZS. Mechanistic research: Selenium regulates virulence factors, reducing adhesion ability and inflammatory damage of Helicobacter pylori. World J Gastroenterol 2024; 30(1): 91-107
URL: https://www.wjgnet.com/1007-9327/full/v30/i1/91.htm
DOI: https://dx.doi.org/10.3748/wjg.v30.i1.91