Determination of β-glucuronidase in human colorectal carcinoma cell lines

Table 1 Conditions for measuring β-glucuronidase in culture medium

Medium (mL)	Water (mL)	Acetate buffer (mL)	pH	Substrate final	Molarity (mL)	Incubation time at 37 °C (h)	Alkalinizing reagent (mL)	Final pH	Coloremeter wavelength (nm)
0.2	0.4	0.2	4.5	0.2	0.006	18	21 + 3 mL H2O	10.2	540

¹Glycine-Duponal reagent: 15.01 g of glycine dissolved in 900 mL of H₂O and brought to pH 11.7 by addition of 50% NaOH solution. Duponal (sodium lauryl sulfate) was added to produce a final concentration of 0.2% and water was added to achieve a final volume of 1 L.