Sequencing of hepatitis C virus cDNA with polymerase chain reaction directed sequencing

Figure 1 Effect of different methods recovering a template on sequencing. A: The first stage PCR products with lower concentration dNTPs and primers as a sequencing template; B: The second stage PCR products directly sequenced without purification; C: Sequencing of PCR amplified cDNA recovered by PEG; D: PCR products purified by agarose gel electrophoresis before sequencing; E:Sequencing of PCR products purified by PAG electrophoresis; F: Sequencing ladders of PCR products purified by agarose gel electrophoresis but without phenol/chloroform extraction.