Adenosine 2A receptor contributes to the facilitation of post-infectious irritable bowel syndrome by γδ T cells via the PKA/CREB/NF-κB signaling pathway

Figure 4 Functional evaluation of γδ T cells in vitro. A: The ATP level changed by differently treated γδ T cells from post-infectious irritable bowel syndrome (PI-IBS) mice. The data was obtained from six mice in each group; B: The proliferation percent of γδ T cells from PI-IBS mouse was analysed through CCK8 assay. The samples were taken from six mice in each group; C: The apoptosis percent of γδ T cells from PI-IBS mouse measured by fluorescence-activated cell sorting. The γδ T cells were taken from six mice in each group; D: Apoptosis rates were detected according to Annexin V and PI double-staining method; E: The cytokines level of IL-1β, IL-6, IL-17A and IFN-α from PI-IBS mice after reinfusing control γδ T cells and γδ T cells treated with CGS21680 or SCH58261, respectively. The data was obtained from six mice in each group. PI-IBS: Post-infectious irritable bowel syndrome. ^aP < 0.05, ^bP < 0.01, ^eP < 0.001 vs the γδ T cell group.