Adenosine 2A receptor contributes to the facilitation of post-infectious irritable bowel syndrome by γδ T cells via the PKA/CREB/NF-κB signaling pathway

Figure 3 γδ T cells’ isolation and functional evaluation. A-C: Based on the results of fluorescence-activated cell sorting, γδ T cells were effectively extracted and purified. P2, unpurified γδ T cells; P3, purified γδ T cells; D-G: Immunohistochemistry labeling of adenosine 2A receptor (A2AR) expression in γδT cells. Scale bars, 100 μm; H: Western blot analysis of A2AR expression levels in γδ T cells. β-Actin is used as the loading control; I: The quantitive result of A2AR expression level in γδ T cells was analyzed from data shown in H. Results from three times of independently repeated experiments were analysed; J: Reverse transcription polymerase chain reaction analysis of the expression level of A2AR mRNA in γδ T cells. The results were independently repeated three times. A2AR: Adenosine 2A receptor. ^aP < 0.05, ^bP < 0.01 vs the γδ T cell group.