Stress granules inhibit endoplasmic reticulum stress-mediated apoptosis during hypoxia-induced injury in acute liver failure

Figure 2 Effects of stress granules agonist on endoplasmic reticulum stress and apoptosis in hepatocyte hypoxia model. A and B: The level of apoptosis was detected by flow cytometry; C and D: The detection kits were used to evaluate the content of HIF-1α in homogenates, lactate dehydrogenase in cell supernatant; E: The expression of G3BP1 in cells was detected by immunofluorescence; F: The expression of TIA-1 in cells was detected by immunofluorescence; G: The histogram displays the amount of molecules expression; H-J: The protein expression levels of G3BP1, TIA-1, ATF4, CHOP, BAX, BCL2, c-cas3 and c-cas9 was detected by western blot. n = 3. Data was shown mean ± SD. ^aP < 0.05 vs normal group; ^bP < 0.05 vs hypoxia group.