Impact of endothelial nitric oxide synthase activation on accelerated liver regeneration in a rat ALPPS model

Figure 5 Western blotting of Akt-endothelial nitric oxide synthase pathway-related proteins. A: Western blotting was used to evaluate the expression of phosphorylated Akt and endothelial nitric oxide synthase (eNOS) in right side of the median lobe at 1, 4, and 6 h after surgery in portal vein ligation (PVL) and associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) groups; B: Comparison of the expression of P-Akt Ser⁴⁷³ and P-eNOS Ser¹¹⁷⁷ in PVL and ALPPS groups (quantification of western blots, n = 5 for each group). Values are expressed as the mean ± SD; n = 5 for each group; ^aP < 0.05; NS: Not significant; eNOS: Endothelial nitric oxide synthase; PVL: Portal vein ligation; ALPPS: Associating liver partition and portal vein ligation for staged hepatectomy.