Single-cell analysis identifies phospholysine phosphohistidine inorganic pyrophosphate phosphatase as a target in ulcerative colitis

Figure 4 Phospholysine phosphohistidine inorganic pyrophosphate phosphatase was identified as a core regulator for ulcerative colitis. A: Venn plot showing the overlap between differential expression genes and public ulcerative colitis data; B: Scatter plot showing the correlation of expression levels between phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) and signal transducer and activator of transcription 3 (STAT3); C: Scatter plot showing the correlation of expression levels between LHPP and FOXP1; D: HE stained colon biopsy samples from healthy individuals and patients diagnosed with ulcerative colitis (UC). Representative images were captured at × 20 magnification. Black arrows: Increased chronic inflammatory infiltrate; E: The relative mRNA level of STAT3, FOXP1, IL6, IL1A, CRP, LHPP, and PCK1 was detected in 12 paired heathy and UC patient tissues; F: Western blot verifying differential STAT3 and LHHP expression in heathy and UC patient tissues; G: The relative expression analysis of STAT3 and LHHP in heathy and UC patient tissues; H: Enzyme-linked immunosorbent assay analysis of IL6 and LHPP levels in serum of heathy and UC patient.