Pien Tze Huang alleviates Concanavalin A-induced autoimmune hepatitis by regulating intestinal microbiota and memory regulatory T cells

Figure 5 Pien Tze Huang regulates the number of T helper type 17 and regulatory T cells in mice with autoimmune hepatitis. A: Mononuclear cells in peripheral blood; B: CD4⁺ lymphocytes measured by flow cytometry; C: CD4⁺IL-17A⁺(T helper type 17) cells. C1-C4: Control, Concanavalin A (Con A), Con A + middle-dose Pien Tze Huang (PTH-M), and Con A + dexamethasone (DXM) group, respectively; C5: Statistical analysis of CD4⁺IL17A⁺ cell frequencies in these four groups; D: CD4⁺CD25⁺Foxp3⁺ cells. D1–D4: Control, Con A, Con A + PTH-M, and Con A + DXM group, respectively; D5: Statistical analysis of CD4⁺CD25⁺Foxp3⁺ cell frequencies in these four groups; E: CD4⁺CD25⁺Foxp3⁺PD-L1⁺ cells. E1–E4: Control, Con A, Con A + PTH-M, and Con A + DXM group, respectively; E5: Statistical analysis of CD4⁺CD25⁺Foxp3⁺PD-L1⁺ cell frequencies in these four groups. Data are representative images or the mean ± SEM (n = 8). ^aP < 0.05 and ^bP < 0.01 vs the control group; ^cP < 0.05 and ^dP < 0.01 vs the Concanavalin A group. IL: Interleukin; ConA: Concanavalin A; PTH: Pien Tze Huang; DXM: Dexamethasone.