Pien Tze Huang alleviates Concanavalin A-induced autoimmune hepatitis by regulating intestinal microbiota and memory regulatory T cells

Figure 1 Dose screening of Pien Tze Huang relieved liver injury induced by Concanavalin A in mice. A: Liver weigh; B: Liver weigh index; C: Spleen weigh; D: Colonic weigh; E: Colonic weigh index; F: Serum alanine aminotransferase; G: Serum aspartate aminotransferase; H-J: The levels of cytokines: Interleukin (IL)-4 (H), IL-10 (I), IL-17A (J) were measured by enzyme-linked immunosorbent assay; K: Representative photographs of hematoxylin and eosin-stained liver sections; L: Scores for liver injury in different groups. According to the Ishak scoring system, liver injury was scored with respect to portal and intralobular inflammation, fragmented necrosis, and bridging necrosis. The extent of the pathology was scored from 0 (no pathology) to 4 (severe pathology). Data are representative images or the mean ± SEM (n = 8). ^aP < 0.05 and ^bP < 0.01 vs the control group; ^cP < 0.05 and ^dP < 0.01 vs the Concanavalin A group. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; IL: Interleukin; ConA: Concanavalin A; PTH: Pien Tze Huang; DXM: Dexamethasone.