Thioridazine reverses trastuzumab resistance in gastric cancer by inhibiting S-phase kinase associated protein 2-mediated aerobic glycolysis

Figure 5 The combination of thioridazine and lapatinib exhibits more pronounced antitumor activity than either alone in vitro against trastuzumab-resistant gastric cancercells. A-C: To HGC27-R, SGC7901-R, and NCI-N87-R cells were treated with trastuzumab (10 μg/mL) or thioridazine (5 μM) alone or in combination for 48 h. Proliferation activity was evaluated by cell counting kit-8 assays; D-F: HGC27-R cells were treated with lapatinib (1 μM or 2 μM) in the presence or absence of thioridazine (5 μM) for 24 h, followed by an apoptosis assay. Similar experiments were performed and results were quantified in SGC7901-R cells. The experiments were repeated three times. ^bP < 0.01. Thior: Thioridazine; Lap: Lapatinib.