First report on establishment and characterization of the extrahepatic cholangiocarcinoma sarcoma cell line CBC2T-2

Figure 2 Morphological features, doubling time and cell cycle of CBC2T-1 cells. A: Bright field morphology of CBC2T-2 cells in the primary,1^st, 10^th, 20^th and 50^th passages (scale bars, 100 μm); B: SEM and TEM electron microscopy were used to observe the cellular ultrastructure (scale bars, 100, 30, 10 μm); C: Flow cytometry of CBC2T-2 cells; D and E: Cumulative growth curve of CBC2T-2 cells.