Prostaglandin F2α synthase promotes oxaliplatin resistance in colorectal cancer through prostaglandin F2α-dependent and F2α-independent mechanism

Figure 7 Prostaglandin F_2α synthase may suppresses the formation of platinum-DNA adducts. A: The effect of Prostaglandin F_2α synthase (PGF2α) (PGFS) overexpression on the formation of platinum-DNA adducts was determined using inductively coupled plasma mass spectrometry in colorectal cancer (CRC) cells; B: The effect of PGFS knockdown on the formation of platinum-DNA adducts in oxaliplatin-resistant (OR) CRC cells; C: The effect of PGF2α on the formation of platinum-DNA adducts in OR CRC cells; D: The reactive oxygen species (ROS) content were significantly decreased in OR CRC cells; E: PGFS overexpression significantly decreased ROS content in CRC cells; F: PGFS knockdown significantly decreased ROS content in OR CRC cells; G: PGFS knockdown significantly decreased ROS content in OR CRC cells (scale bar, 50 μm). ^aP < 0.05, ^bP < 0.01; ^cNo significance. PGF_2α: Prostaglandin F_2α; Oxa: Oxaliplatin.