Prostaglandin F2α synthase promotes oxaliplatin resistance in colorectal cancer through prostaglandin F2α-dependent and F2α-independent mechanism

Figure 6 The inhibitory effect of prostaglandin F_2α on oxaliplatin-induced cytotoxicity. A: Western blots showed the effect of prostaglandin F2α synthase (PGF_2α) (PGFS) on the expressions of proliferating cell nuclear antigen, cleaved-poly ADP-ribose polymerase, and γ-H2A histone family member X (γ-H2AX) in PGFS knockdown, oxaliplatin-resistant (OR) cells; B: The effect of PGF2α on apoptosis in PGFS knockdown, OR cells evaluated by immunofluorescence. scale bar 100 uM; C: The effect of PGF2α on the cleavage of γ-H2AX protein expressions in PGFS knockdown, OR cells; D: The effect of PGF2α on DNA damage in PGFs knockdown, OR cells evaluated by single-cell gel electrophoresis. scale bar 50 μm). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dNo significance. PCNA: Proliferating cell nuclear antigen; PARP: Poly ADP-ribose polymerase; γ-H2AX: γ-H2A histone family member X; TUNEL: Terminal deoxynucleotidyl transferase dUTP nick end labeling.