Prostaglandin F2α synthase promotes oxaliplatin resistance in colorectal cancer through prostaglandin F2α-dependent and F2α-independent mechanism

Figure 4 The protective effect of prostaglandin F_2α synthase on DNA damage induced by oxaliplatin in colorectal cancer cells. A and B: DNA damage marker protein γ-H2A histone family member X (γ-H2AX) was determined using western blot in HCT116 and HCT8 cells and HCT116-OxR and HCT8-OxR cells; C: γ-H2AX fluorescent spots were detected using immunofluorescence staining; D and E: Detection of DNA damage using single-cell gel electrophoresis (comet assay) in parent and drug-resistant cells (scale bar, 50 μm). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. PGFS: Prostaglandin F_2α synthase; Oxa: Oxaliplatin; γ-H2AX: γ-H2A histone family member X.