Green tea polyphenols alleviate di-(2-ethylhexyl) phthalate-induced liver injury in mice

Figure 10  Construction of the microRNA-mRNA-protein regulatory axis. A: The network diagram was constructed using differentially expressed mRNAs and microRNAs (miRNAs); B: Wayne diagram indicating the intersection of the miRNA-mRNA regulatory axis and mouse interaction network from the starBase database; C: Correlation analyses of mmu-miR-141-3p and Zcchc24; D: Correlation analyses of mmu-miR-9-5p and Zbtb7a; E: Western blotting validation of Zcchc24 protein expression in the control, model, and treatment groups; F: Semiquantitative analysis of Zcchc24 protein levels in the control, model, and treatment groups; G: The Sankey plot of the miRNA-mRNA-protein regulatory axes mediating the suppressive effects of green tea polyphenols (GTPs) on di-(2-ethylhexyl) phthalate (DEHP)-induced liver damage in mice. Red and blue fonts indicate upregulated and downregulated expression, respectively. ^dP < 0.01 (compared to DEHP group). DEHP: Di (2-ethylhexyl) phthalate; GTPs: Green tea polyphenols.