Angiotensin-converting enzyme 2 improves liver fibrosis in mice by regulating autophagy of hepatic stellate cells

Figure 3 Effect of rAAV-Angiotensin-converting enzyme 2 treatment on the expression of Alpha-smooth muscle actin, Fibronectin and CD31 in CCl₄-induced fibrotic mice was evaluated by immunohistochemistry staining (magnification × 200). A: The expression level of Alpha-smooth muscle actin in the liver tissues of each group was detected; B: The expression level of Fibronectin in the liver tissues of each group was detected; C: The expression level of CD31 in the liver tissues of each group was detected; D-F: The relative expression levels of the three proteins in each group were analyzed using Image-Pro Plus 6.0. ^aP < 0.01 vs Control; ^bP < 0.05 vs CCl₄; ^cP < 0.01 vs rAAV-ACE2 + CCl₄; ^dP < 0.001 vs Control; ^eP < 0.001 vs CCl_4. Rapa: Rapamycin; α-SMA: Alpha-smooth muscle actin; FN: Fibronectin; ACE2: Angiotensin-converting enzyme 2.