Poly(A)-specific ribonuclease protein promotes the proliferation, invasion and migration of esophageal cancer cells

Figure 3 Effect of poly(A)-specific ribonuclease on esophageal cancer cell proliferation, migration and invasion, cell cycle, and cell apoptosis in vitro. A and B: The interference efficiency of poly(A)-specific ribonuclease (PARN) via shRNA; C: Representative images of the Celigo cell count assay (magnification, × 100) and cumulative data of cell numbers in TE-1 and Eca-109 cells transfected with control shRNA or PARN shRNA; D: Representative images and statistical analysis of the wound healing assay; E: Representative images and statistical analysis of the transwell assay; F and G: Representative images and statistical analysis of flow cytometry analysis of TE-1 and Eca-109 cells after transfection. Cell cycle analysis revealed that PARN affected TE-1 and Eca-109 cell proliferation (F), and cell apoptosis analysis showed that PARN affected the apoptosis of TE-1 and Eca-109 cells (G). ^aP < 0.05, ^bP < 0.01, ^aP < 0.0001, ^cP < 0.001, means ± SDs are shown. sh PARN: Poly(A)-specific ribonuclease shRNA; sh-NC: Control shRNA.