Huangqin decoction alleviates lipid metabolism disorders and insulin resistance in nonalcoholic fatty liver disease by triggering Sirt1/NF-κB pathway

Figure 6 Huangqin decoction improves lipotoxicity, inflammation, and insulin resistance in palmitic acid-induced HepG2 cells. Data are presented as mean ± SD of at least three independent experiments. ^aP < 0.01 vs the control group, ^bP < 0.05 and ^cP < 0.01 vs the palmitic acid (PA) group. A: Viability of HepG2 cells following incubation with different concentrations of Huangqin decoction (HQD); B: Viability of HepG2 cells following co-culture with PA and different concentrations of HQD; C and D: Levels of triacylglycerol and total cholesterol in HepG2 cells; E: Representative micrographs of Oil Red O staining of HepG2 cells; F–H: Levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor-α in HepG2 cells; I: Level of glucose uptake in HepG2 cells. 2DG: 2-deoxydglucose; HQD, Huangqin decoction; IL: Interleukin; TC: Total cholesterol; TG: Triacylglycerol; TNF: Tumor necrosis factor; PA: Palmitic acid.