Antagonizing adipose tissue-derived exosome miR-103-hepatocyte phosphatase and tensin homolog pathway alleviates autophagy in non-alcoholic steatohepatitis: A trans-cellular crosstalk

Figure 3 Invitro experiments confirmed the interaction between miR-103 and phosphatase and tensin homolog gene. A: Oil-red staining of cells from different groups showed different lipid droplet accumulation and inflammatory cell infiltration; B: The results of ELISA to measure serum alanine aminotransferase, aspartate aminotransferase, superoxide dismutase and malondialdehyde; C: Western blotting detected the expression of the autophagy-related protein of cells from different groups; D: Transmission electron microscopy pictures of autophagosomes (red arrowhead) in cells from different groups. ^aP < 0.05, ^bP < 0.01, ns: No significance. NASH: Non-alcoholic steatohepatitis; PTEN: Phosphatase and tensin homolog; NC: Negative control; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; TG: Total triglycerides; CHOL: Cholesterol; SOD: Superoxide dismutase; MDA: Malondialdehyde.