F-box only protein 2 exacerbates non-alcoholic fatty liver disease by targeting the hydroxyl CoA dehydrogenase alpha subunit

Figure 5 F-box only protein 2 directly binds to the hydroxyl CoA dehydrogenase alpha subunit and vice versa. A: Proteins interacting with endogenous F-box only protein 2 (FBXO2) in HepG2 cells as revealed using a co-immunoprecipitation (co-IP)-based mass spectrometry method; B: Immunofluorescence staining of HEK293T cells transfected with His-FBXO2 and Myc-the hydroxyl CoA dehydrogenase alpha subunit plasmids; C-F: Co-IP assays in HEK293T and HepG2 cells. FBXO2: F-box only protein 2; HADHA: Hydroxyl CoA dehydrogenase alpha subunit; IgG: Immunoglobulin G; DAPI: 4’-6-diamidino-2-phenylindole.