F-box only protein 2 exacerbates non-alcoholic fatty liver disease by targeting the hydroxyl CoA dehydrogenase alpha subunit

Figure 2 F-box only protein 2 expression was up-regulated in livers of non-alcoholic fatty liver disease patients and high-fat diet mice models. A and B: Representative images of hematoxylin and eosin (H&E) and Oil Red O staining of liver tissues obtained from healthy controls and patients with non-alcoholic fatty liver disease (NAFLD). Scale bars, 50 μm and 100 μm; C: Western blot of F-box only protein 2 (FBXO2) expression in the liver tissues of healthy controls and patients with NAFLD (n = 10 liver samples per group); D: Expression of FBXO2 in liver tissue obtained from healthy controls and patients with NAFLD (n = 10 liver samples per group); E: Representative images of immunohistochemical staining of FBXO2 in liver tissue obtained from healthy controls and patients with NAFLD. Scale bars, 50 μm; F and G: Representative images of H&E and Oil Red O staining of liver tissue isolated from C57BL/6J mice treated with a normal chow diet (ND) or high-fat diet (HFD) for 16 wk. Scale bars, 50 μm and 100 μm; H: Representative images of FBXO2 western blots from liver tissue of C57BL/6J mice treated with an ND or HFD for 16 wk; I: Expression of FBXO2 in liver tissue obtained from C57BL/6J mice treated with an ND or HFD for 16 wk as detected using quantitative reverse transcription polymerase chain reaction (n = 8 liver samples per group); J: Representative images of immunohistochemical staining of FBXO2 in liver tissue obtained from C57BL/6J mice treated with an ND or HFD for 16 wk. Scale bars, 50 μm. ^bP < 0.01, ^dP < 0.0001. FBXO2: F-box only protein 2; NAFLD: Non-alcoholic fatty liver disease; HFD: High-fat diet; H&E: Hematoxylin and eosin; ND: Normal chow diet.