Transcriptome sequencing and experiments reveal the effect of formyl peptide receptor 2 on liver homeostasis

Figure 4 The effect of formyl peptide receptor 2 deletion on cell cycle. A: Quantitative real time-polymerase chain reaction (qRT-PCR) validates the expression of key cell cycle genes regulated by formyl peptide receptor 2 (Fpr2), including CycA, CycB1, Cdc20, Cdc25c, and Mps1 (n = 6). P values are indicated on the graphs; B: qRT-PCR and western blot analyses are used to validate the changes in expression of CDK1; C: The effect of different concentrations of WRW4 on the proliferation activity of HepG2 cells detected by CCK-8 (n = 5); D: The cell cycle distribution of HepG2 cells treated with WRW4 (1 μM) and dimethyl sulfoxide determined by flow cytometry (n = 3). Data are shown as mean ± SD. ^aP < 0.0001 vs the 0 μM group; ^bP < 0.05 vs the dimethyl sulfoxide group; NS: No significant; Fpr2: Formyl peptide receptor 2; WT: Wild-type; DMSO: Dimethyl sulfoxide.