Mechanism of ELL-associated factor 2 and vasohibin 1 regulating invasion, migration, and angiogenesis in colorectal cancer

Figure 8 Overexpressing ELL-associated factor 2 inhibits tumor-induced angiogenesis in RKO cells via transforming growth factor-β1 related pathway. A: Conditioned medium from RKO cells transfected with ELL-associated factor 2-overexpressed plasmids or treated with transforming growth factor-β1 recombinant protein was collected to perform tube formation assay; B and C: The migration ability of Human Umbilical Vein Endothelial Cells was analyzed by transwell migration assay (B) and wound healing assay (C). And the results were measured by ImageJ software. ^aP < 0.05, ^bP < 0.001. EAF2: ELL-associated factor 2; TGF-β1: Transforming growth factor-β1.