BMI-1 activates hepatic stellate cells to promote the epithelial-mesenchymal transition of colorectal cancer cells

Figure 3 Effects of BMI-1 overexpressed hepatic stellate cell conditioned medium on cell proliferation and migration of colorectal cancer cells. A: Viability of HCT116 and DLD-1 cells cultured in conditioned medium (CM) from negative control (NC) LX2 (NC CM) or CM from BMI-1 overexpressed LX2 (BMI-1 CM) was confirmed by the Cell Counting Kit-8 assay; B: Colony formation assay of colorectal cancer (CRC) cells cultured in NC CM or BMI-1 CM; C: Wound healing assay of CRC cells cultured in NC CM or BMI-1 CM, the healing percentages were calculated at 24 h and 48 h; D: Transwell migration assay of CRC cells cultured in NC CM or BMI-1 CM, the numbers of cells which migrated were counted; E: Quantitative polymerase chain reaction assay of the expression of epithelial-mesenchymal transition (EMT)-related markers in CRC cells cultured in NC CM or BMI-1 CM; F: Western blot assays of the expression of EMT-related markers in CRC cells cultured in NC CM or BMI-1 CM; G: Immunofluorescence analysis showed that vimentin and Snail expression levels were increased in CRC cells cultured in BMI-1 CM. ^bP < 0.01, ^cP < 0.001 vs NC CM. DAPI: 4',6-diamidino-2-phenylindole.