BMI-1 activates hepatic stellate cells to promote the epithelial-mesenchymal transition of colorectal cancer cells

Figure 1 BMI-1 expression increases in liver cells during colorectal cancer liver metastasis. A: Immunohistochemistry analysis of BMI-1 in liver metastasis and paired liver tissues of colorectal cancer liver metastasis (CRLM) patients; B: Spleens and livers of mice were photographed after intrasplenic injection of CT26 cells at the indicated times; C: Western blot analysis of BMI-1 expression in mouse livers (n = 3); D: Quantitative protein expression of BMI-1 normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in mouse livers (n = 3); E: Quantitative polymerase chain reaction detection of BMI-1 expression in mouse livers (n = 3); ^cP < 0.001 vs D0; F: Immunohistochemistry confirmed that BMI-1 expression was increased in mouse livers during CRLM.