Plasma exosomal hsa_circ_0079439 as a novel biomarker for early detection of gastric cancer

Figure 2 Identification of exosomes and bioinformatic analysis of exosomal circular RNAs. A: Exosomes were observed by transmission electron microscopy. The scale bar is 200 nm; B: Diameter and quantity of the exosomes; C: The positive markers of exosomes (CD9, TSG101, and CD81) and the negative marker (Calnexin) were tested by Western blot; D: Clustering analysis of circular RNAs (circRNAs); E: Kyoto Encyclopedia of Genes and Genomes pathway analysis of cluster 5 of differently expressed circRNAs; F: Gene Ontology enrichment analysis of cluster 5 of differently expressed circRNAs. HD: Healthy donor; GC: Gastric cancer; EGC: Early gastric cancer; LAGC: Locally advanced gastric cancer; MGC: Metastatic gastric cancer; AGC: Advanced gastric cancer; BP: Biological process; CC: Cell component; MF: Molecular function.