Study of the roles of caspase-3 and nuclear factor kappa B in myenteric neurons in a P2X7 receptor knockout mouse model of ulcerative colitis

Figure 4 Colocalization of calretinin (red) with total caspase-3 (green) and DAPI (blue) in neurons in the myenteric plexus of the distal colon in mice of different groups. A1-A3: Wild-type (WT) sham 24 h; B1-B3: WT colitis 24 h; C1-C3: Knockout (KO) sham 24 h; D1-D3: KO colitis 24 h; E1-E3: WT sham 4 d; F1-F3: WT colitis 4 d; G1-G3: KO sham 4 d; and H1-H3: KO colitis 4 d groups. The single arrows indicate calretinin-ir (A1-H1) and total caspase-3-ir (A2-H2) neurons, and the double arrows indicate the colocalization of calretinin with total caspase-3 and DAPI (A3-H3). Scale bar = 30 µm. WT: Wild-type; KO: Knockout.