Study of the roles of caspase-3 and nuclear factor kappa B in myenteric neurons in a P2X7 receptor knockout mouse model of ulcerative colitis

Table 3 Characteristics of primary and secondary antibodies

Antigen	Host	Dilution	Source
Calretinin	Goat	1:300	Swant
P2X7 receptor	Rabbit	1:200	Merck Millipore
Cleaved caspase-3	Rabbit	1:100	Cell Signaling
Total caspase-3	Rabbit	1:100	Cell Signaling
Phospho- NF-κB	Rabbit	1:100	Cell Signaling
Total NF-κB (p65)	Rabbit	1:100	Cell Signaling
Secondary antibodies
Alexa Fluor 488-conjugated donkey anti-rabbit IgG	Donkey	1:500	Molecular Probes
Alexa Fluor 594-conjugated donkey anti-sheep IgG	Donkey	1:100	Molecular Probes

NF-κB: Nuclear factor kappa B.