TATA-box-binding protein-associated factor 15 is a novel biomarker that promotes cell proliferation and migration in gastrointestinal stromal tumor

Figure 3 TATA-box-binding protein-associated factor 15 regulated the proliferation and migration of gastrointestinal stromal tumor cells. A and B: Cell counting kit-8 assay was performed to detect the proliferation of gastrointestinal stromal tumor (GIST)-882 and GIST-T1 after cell transfection. P values were calculated using the Student’s t-test; C and D: A colony formation assay identified the proliferative ability of GIST-882 and GIST-T1 after cell transfection. Statistical analyses were performed using the Student’s t-test; E-H: A transwell assay was performed to analyze the migratory and invasive ability of GIST-882 and GIST-T1 cells after transfection. P values were calculated using the Student’s t-test; I-K: A wound healing assay was conducted to confirm the migratory ability of GIST-882 and GIST-T1 cells after transfection. P values were calculated using the Student’s t-test. ^aP < 0.05, ^bP < 0.01. GIST: Gastrointestinal stromal tumor; NS: No significance between groups; sh2: shRNA2 targeting TATA-box-binding protein-associated factor 15; sh3: shRNA3 targeting TATA-box-binding protein-associated factor 15; scr: Scrambled shRNA; CCK8: Cell counting kit-8.