TATA-box-binding protein-associated factor 15 is a novel biomarker that promotes cell proliferation and migration in gastrointestinal stromal tumor

Figure 2 The knockdown efficiency of short hairpin-TATA-box-binding protein-associated factor 15 in gastrointestinal stromal tumor cell lines. A: Western blotting to detect the expression level of TATA-box-binding protein-associated factor 15 (TAF15) in gastrointestinal stromal tumor(GIST) cell lines. P values were calculated by one-way analysis of variance; B and C: TAF15 was knocked down using three short hairpin (sh) RNAs (sh1, sh2, and sh3) and scrambled shRNA (scr) as control in GIST-882 and GIST-T1 cells. The results showed that sh2 and sh3 could reduce the expression level of TAF15 significantly when compared to scr as tested by western blotting analysis; D and E: The fluorescence intensity of TAF15 in sh2 and sh3 was reduced significantly when compared to scr in GIST-882 and GIST-T1 cells as tested by a cell immunofluorescence assay. P values were calculated using the Student’s t-test. ^aP < 0.05, ^bP < 0.01. NS: No significance between groups; GIST: Gastrointestinal stromal tumor; TAF15: TATA-box-binding protein-associated factor 15; sh1: shRNA1 targeting TATA-box-binding protein-associated factor 15; sh2: shRNA2 targeting TATA-box-binding protein-associated factor 15; sh3: shRNA3 targeting TATA-box-binding protein-associated factor 15; scr: Scrambled shRNA.