Susceptibility patterns and virulence genotypes of Helicobacter pylori affecting eradication therapy outcomes among Egyptian patients with gastroduodenal diseases

doi:10.3748/wjg.v29.i19.2950

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May 21, 2023 (publication date) through Jan 20, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 21, 2023; 29(19): 2950-2960
Published online May 21, 2023. doi: 10.3748/wjg.v29.i19.2950

Table 1 Primers used in this study for multiplex polymerase chain reaction for detection of vacA and cagA genotypes in Helicobacter pylori isolates

Primer	Nucleotide sequence, 5'—3'	Gene	Size, bp
vacA
VA1-F	ATGGAAATACAACAAACACAC	vacA s1/s2	259/286
VA1-R	CTGCTTGAATGCGCCAAAC
VA2-F	CAATCTGTCCAATCAAGCGAG	vacA m1/m2	570/645
VA2-R	GCGTCAAAATAATTCCAAGG
Cag-F	GTTGATAACGCTGTCGCTTC	cagA	349
Cag-R	GGGTTGTATGATATTTTCCATAA

m1/m2: Middle regions; s1/s2: Signal regions.

Citation: Asaad AM, El-Azab G, Abdelsameea E, Elbahr O, Kamal A, Abdel-Samiee M, Abdelfattah A, Abdallah H, Maher D, El-Refaie A, Ghanem SE, Ansari S, Awad SM. Susceptibility patterns and virulence genotypes of Helicobacter pylori affecting eradication therapy outcomes among Egyptian patients with gastroduodenal diseases. World J Gastroenterol 2023; 29(19): 2950-2960
URL: https://www.wjgnet.com/1007-9327/full/v29/i19/2950.htm
DOI: https://dx.doi.org/10.3748/wjg.v29.i19.2950