Calcitriol attenuates liver fibrosis through hepatitis C virus nonstructural protein 3-transactivated protein 1-mediated TGF β1/Smad3 and NF-κB signaling pathways

Figure 2 Influence of nonstructural protein 3-transactivated protein 1 on liver fibrosis in vitro. A: Western blot analysis of fibrosis-related genes after Nonstructural protein 3-transactivated protein 1 (NS3TP1) overexpression (n = 3); B: Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of fibrosis-related genes after NS3TP1 overexpression (n = 3); C: Western blot analysis of the fibrosis-related genes after NS3TP1 interference (n = 3); D: RT-qPCR analysis of the fibrosis-related genes after NS3TP1 interference (n = 3); E and F: Cell proliferation was measured by cell counting kit-8 assays after interference or overexpression of NS3TP1 (n = 3); G and H: Western blot analysis of Bcl-2 and Bax after interference or overexpression of NS3TP1 (n = 3). The data was represented as mean ± SE. ^aP < 0.05, ^bP < 0.01 vs pcDNA-NS3TP1 group; ^cP < 0.05, ^dP < 0.01, ^eP < 0.001 vs siRNA-NS3TP1 group. NS3TP1: Nonstructural protein 3-transactivated protein 1; CCK-8: Cell counting kit-8; COL1A1: Type 1 collagen alpha 1 chain; COL1A2: Type 1 collagen alpha 2 chain; COL3A1: Type 3 collagen alpha 1 chain; COL4A2: Type 4 collagen alpha 2 chain.