Key elements determining the intestinal region-specific environment of enteric neurons in type 1 diabetes

Figure 1 Representative fluorescent micrograph of a paraffin section of intestinal wall from control rat colon after Protein gene product 9. 5 immunohistochemistry. Protein gene product 9.5 (PGP9.5) (green) was used as a pan-neuronal marker to label neuronal elements and 4',6-diamidino-2-phenylindole (DAPI) (blue) labelled nuclei. A: DAPI; B: PGP9.5; C: DAPI + PGP9.5. M: mucosal layer, SM: Submucosal layer; CM: Circular muscle; LM: Longitudinal muscle; White arrows: Myenteric ganglia, Yellow arrows: Submucous ganglia; PGP9.5: Protein gene product 9.5; DAPI: 4',6-diamidino-2-phenylindole; Scale bar: 200 μm.