Ferroptosis inhibition attenuates inflammatory response in mice with acute hypertriglyceridemic pancreatitis

Figure 5 The inhibition of NADPH oxidase 2 attenuated hypertriglyceridemic pancreatitis through a ferroptosis reduction in mice (n = 4). A: Kyoto Encyclopedia of Genes and Genomes pathways enriched with protein NADPH oxidase (NOX) 2 in comparison of P-407 + caerulein (CAE) group vs CAE group; B: Volcano plot showing the location of NOX2 in comparison of P-407 + CAE group vs CAE group; C: Immunohistochemistry (IHC) for NOX2 in the pancreas tissues of the P-407 + CAE and P-407 + CAE + Vas2870 (Vas) groups; D: The malonaldehyde levels; E: The glutathione levels; F: Fe²⁺ levels in the pancreases of the mice in the P-407 + CAE and P-407 + CAE + Vas groups; G: IHC for GPX4 in the pancreas tissues of the P-407 + CAE and P-407 + CAE + Vas groups; H: Hematoxylin–eosin (HE) staining and histological scores of the pancreas; I: HE staining and histological scores of the lung; J: HE staining and histological scores of the kidney; K: Serum TNF-α, IL-1β, and IL-6 levels of the P-407 + CAE and P-407 + CAE + Vas groups. The scale bar represents 50 mm. NOX: NADPH oxidase; ROS: Reactive oxygen species; CAE: Caerulein; Vas: Vas2870; MDA: Malonaldehyde; GSH: Glutathione; GPX4: Glutathione peroxidase 4; TNF: Tumor necrosis factor; IL: Interleukin.