Effect and mechanism of reactive oxygen species-mediated NOD-like receptor family pyrin domain-containing 3 inflammasome activation in hepatic alveolar echinococcosis

Figure 5 Reactive oxygen species-mediated NOD-like receptor family pyrin domain-containing 3-caspase-1-interleukin-18 pathway activation in Kupffer cells. A: Reactive oxygen species (ROS) production with the indicated N-acetyl-L-cysteine (NAC) dose at the specified time points. ^bP < 0.01, blank vs 5 mmol/L; ^aP < 0.05, 10 mmol/L vs 5 mmol/L. ^bP < 0.0120 mmol/L vs 5 mmol/L; B: Representation of the Transwell model used in this study; C: ROS production in the co-culture groups treated with or without NAC at the indicated time points; D: Relative production of ROS; E: Interleukin (IL)-18 expression; F: IL-1β expression; G: Apoptosis of hepatocytes in the co-culture groups treated with or without NAC at 24, 48, and 72 h; H: Cell viability; I: Western blotting analysis of the indicated proteins; J: NLRP3 IntDen/β-actin IntDen protein expression; K: Caspase-1 IntDen/β-actin IntDen protein expression. ^aP < 0.05; ^bP < 0.01. NLRP3: NOD-like receptor family pyrin domain-containing 3; ROS: Reactive oxygen species; FITC-A: Fluorescein isothiocyanate isomer I-A; PI: Propidium Iodide; NAC: N-acetyl-L-cysteine; IL-1β: Interleukin-1β; IL-18: Interleukin-18.