Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269I

Figure 4 Confirmation of multiprobe locked nucleic acid real-time polymerase chain reaction identification results of hepatitis B virus rtL269 variants by direct sequencing. Nucleotide bases are shown in the parentheses. Lowercase letters represent the base present in a lower amount relative to the dominant variant. Bold indicates the dominant amino acids and bases. Arrows represent the codon sequence positions for leucine or isoleucine; yellow, mixed bases.