Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269I

Table 4 Samples which cannot be identified by locked nucleic acid real-time polymerase chain reaction assay

No.	Patients	Direct sequencing (AAACTCAARCAATGT)	Type	LNA-RT-PCR
1	SNU3 30 HCC	AAAATCAAGCACTGT	I	Not detected
2	SNU3 70 HCC	AAAATTAAGCAATGT	I	Not detected
3	SNU3 82 CH	AAAATCAAACTATGT	I	Not detected
4	SNU3 123 HCC	AAACTTAAGCAATGT	L	Not detected
5	SNU3 31 CH	AAAATCCAGCAATGT	I	Not detected
6	SNU3 355 LC	AAAATTAAGCAATG	I	Not detected
7	SNU3 388 LC	AAACTTAAGCAATGT	L	Not detected

Bases in bold indicate the different ones from the target probe sequence. LNA-RT-PCR: Locked nucleic acid real-time polymerase chain reaction; HCC: Hepatocellular carcinoma; CH: Chronic hepatitis; LC: Liver cirrhosis.