In vivo recognition of bioactive substances of Polygonum multiflorum for protecting mitochondria against metabolic dysfunction-associated fatty liver disease

Figure 9 Effects of Polygonum multiflorum-derived monomer group in liver mitochondria on the liver lipid content of high-fat diet-fed mice. Mice were administered saline, Polygonum multiflorum-derived monomer groups (30, and 90 mg/kg/d), or resveratrol (18 mg/kg/d). After 3 mo of high-fat diet feeding, mice were sacrificed, and livers were collected. A: Liver histology after Oil Red O staining was observed under a light microscope (× 200); B: Liver levels of total cholesterol were determined using a commercial kit; C: Liver levels of alanine transaminase were determined using a commercial kit; D: Liver levels of aspartate transaminase were determined using a commercial kit. Values represent the mean ± SD from six animals. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs the relevant model group. ALT: Alanine transaminase; AST: Aspartate transaminase; HMG: High-dose Polygonum multiflorum-derived monomer group; LMG: Low-dose Polygonum multiflorum-derived monomer group; MOD: Model; NC: Normal control; RES: Resveratrol; TC: Total cholesterol.